Low pH Stress Enhances Gluconic Acid Accumulation with Enzymatic Hydrolysate as Feedstock Using Gluconobacter oxydans

Gluconic acid has been increasingly in demand recent years due to the wide applications food, healthcare and construction industries. Plant-derived biomass is rich biopolymers that comprise glucose as monomeric unit, which provide abundant feedstock for gluconic production. Gluconobacter oxydans can rapidly incompletely oxidize it regarded ideal industrial microorganism. Once depleted, will be further bio-oxidized 2-ketogluconic by oxydans. The endpoint difficult controlled, especially an fermentation process. In this study, was found low pH environment (2.5~3.5) could limit metabolism of resulted a yield over 95%. Therefore, stress strategy efficiently producing from biomass-derived put forward investigated with enzymatic hydrolysate. As result, 98.8 g/L 96% obtained concentrated corncob hydrolysate initially contained 100 1.4 cells loading addition, effectively control end-point decrease risk microbial contamination. Overall, provides potential production lignocellulosic materials.